BioWDL: gatk-variantcalling

A pipeline that calls variants on a group of BAM files.

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Inputs for GatkVariantCalling

The following is an overview of all available inputs in GatkVariantCalling.

Required inputs

GatkVariantCalling.bamFiles
Array[struct(file : File, index : File, md5sum : String?)] — Default: None
BAM files to be analysed by GATK. The should be recalibrated beforehand if required.
GatkVariantCalling.dbsnpVCF
File — Default: None
dbsnp VCF file used for checking known sites
GatkVariantCalling.dbsnpVCFIndex
File — Default: None
index (.tbi) file for the dbsnp VCF
GatkVariantCalling.referenceFasta
File — Default: None
The reference fasta file
GatkVariantCalling.referenceFastaDict
File — Default: None
Sequence dictionary (.dict) file of the reference
GatkVariantCalling.referenceFastaFai
File — Default: None
Fasta index (.fai) file of the reference

Other common inputs

GatkVariantCalling.outputDir
String — Default: "."
The directory where the output files should be located
GatkVariantCalling.vcfBasename
String — Default: "multisample"
The basename of the VCF and GVCF files that are outputted by the workflow

Advanced inputs

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GatkVariantCalling.dockerImages
Map[String,String] — Default: {"picard": "quay.io/biocontainers/picard:2.20.5--0", "gatk4": "quay.io/biocontainers/gatk4:4.1.0.0--0", "biopet-scatterregions": "quay.io/biocontainers/biopet-scatterregions:0.2--0"}
specify which docker images should be used for running this pipeline

Other inputs

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GatkVariantCalling.gatherGvcfs.intervals
Array[File] — Default: []
???
GatkVariantCalling.gatherGvcfs.javaXmx
String — Default: "12G"
???
GatkVariantCalling.gatherGvcfs.memory
String — Default: "24G"
???
GatkVariantCalling.gatherVcfs.javaXmx
String — Default: "8G"
???
GatkVariantCalling.gatherVcfs.memory
String — Default: "24G"
???
GatkVariantCalling.genotypeGvcfs.javaXmx
String — Default: "6G"
???
GatkVariantCalling.genotypeGvcfs.memory
String — Default: "18G"
???
GatkVariantCalling.Gvcf.haplotypeCallerGvcf.contamination
Float — Default: 0.0
???
GatkVariantCalling.Gvcf.haplotypeCallerGvcf.javaXmx
String — Default: "4G"
???
GatkVariantCalling.Gvcf.haplotypeCallerGvcf.memory
String — Default: "12G"
???
GatkVariantCalling.orderedScatters.dockerImage
String — Default: "python:3.7-slim"
???
GatkVariantCalling.regions
File? — Default: None
???
GatkVariantCalling.scatterList.bamFile
File? — Default: None
???
GatkVariantCalling.scatterList.bamIndex
File? — Default: None
???
GatkVariantCalling.scatterList.javaXmx
String — Default: "8G"
???
GatkVariantCalling.scatterList.memory
String — Default: "24G"
???
GatkVariantCalling.scatterList.notSplitContigs
Boolean — Default: false
???
GatkVariantCalling.scatterSize
Int — Default: 1000000000
???