BioWDL: structural-variantcalling

A workflow for calling structural variants.

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Inputs for SVcalling

The following is an overview of all available inputs in SVcalling.

Required inputs

SVcalling.bamFile
File
sorted BAM file
SVcalling.bamIndex
File
BAM index(.bai) file
SVcalling.bwaIndex
struct(fastaFile : File, indexFiles : Array[File])
Struct containing the BWA reference files
SVcalling.manta.cores
Int — Default: 1
The the number of cores required to run a program
SVcalling.manta.memoryGb
Int — Default: 4
The memory required to run the manta
SVcalling.referenceFasta
File
The reference fasta file
SVcalling.referenceFastaDict
File
Sequence dictionary (.dict) file of the reference
SVcalling.referenceFastaFai
File
Fasta index (.fai) file of the reference
SVcalling.sample
String
The name of the sample

Other common inputs

SVcalling.manta.callRegions
File?
The bed file which indicates the regions to operate on.
SVcalling.manta.callRegionsIndex
File?
The index of the bed file which indicates the regions to operate on.
SVcalling.manta.exome
Boolean — Default: false
Whether or not the data is from exome sequencing.
SVcalling.outputDir
String — Default: "."
The directory the output should be written to.

Advanced inputs

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SVcalling.clever.memory
String — Default: "15G"
The memory required to run the programs
SVcalling.clever.threads
Int — Default: 10
The the number of threads required to run a program
SVcalling.delly.memory
String — Default: "15G"
The memory required to run the programs
SVcalling.dockerImages
Map[String,String] — Default: {"bcftools": "quay.io/biocontainers/bcftools:1.9--ha228f0b_3", "clever": "quay.io/biocontainers/clever-toolkit:2.4--py36hcfe0e84_6", "delly": "quay.io/biocontainers/delly:0.8.1--h4037b6b_1", "manta": "quay.io/biocontainers/manta:1.4.0--py27_1", "picard": "quay.io/biocontainers/picard:2.19.0--0", "samtools": "quay.io/biocontainers/samtools:1.8--h46bd0b3_5", "survivor": "quay.io/biocontainers/survivor:1.0.6--h6bb024c_0"}
A map describing the docker image used for the tasks.
SVcalling.mateclever.cleverMaxDelLength
Int — Default: 100000
The maximum deletion length to look for in Clever predictions.
SVcalling.mateclever.maxLengthDiff
Int — Default: 30
The maximum length difference between split-read and read-pair deletion to be considered identical.
SVcalling.mateclever.maxOffset
Int — Default: 150
The maximum center distance between split-read and read-pair deletion to be considered identical.
SVcalling.mateclever.memory
String — Default: "15G"
The memory required to run the programs
SVcalling.mateclever.threads
Int — Default: 10
The the number of threads required to run a program
SVcalling.renameSample.javaXmx
String — Default: "8G"
The max. memory allocated for JAVA
SVcalling.renameSample.memory
String — Default: "24G"
The memory required to run the programs
SVcalling.survivor.breakpointDistance
Int — Default: 1000
The distance between pairwise breakpoints between SVs
SVcalling.survivor.distanceBySvSize
Int — Default: 0
A boolean to predict the pairwise distance between the SVs based on their size
SVcalling.survivor.memory
String — Default: "24G"
The memory required to run the programs
SVcalling.survivor.minSize
Int — Default: 30
The mimimum size of SV to be merged
SVcalling.survivor.strandType
Int — Default: 1
A boolean to include strand type of an SV to be merged
SVcalling.survivor.suppVecs
Int — Default: 2
The minimum number of SV callers to support the merging
SVcalling.survivor.svType
Int — Default: 1
A boolean to include the type SV to be merged